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Clinical and Laboratory Standards Institute agar well diffusion method
Agar Well Diffusion Method, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/agar+well+diffusion+method/agar+well+diffusion+method/pmc12209781-110-20-24
Average 90 stars, based on 1 article reviews
agar well diffusion method - by Bioz Stars, 2026-10
90/100 stars

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Activity Assay:

Article Title: Evaluation of Biological Potential of Red Onion Skin Extract for Anticancer and Antimicrobial Activities
Article Snippet: Chemotherapy, while effective in combating cancer, frequently induces severe adverse effects and lacks specificity.. This investigation aims to assess the potential of red onion skin, a byproduct abundant in polyphenols and flavonoids, as a reservoir of natural compounds possessing anticancer and antimicrobial properties.. Two extracts, specifically ethanolic red onion skin (EROS) extract, and chromatographic red onion skin (CROS) extract were subjected to comprehensive biological analysis.

Article Title: Removal of tetracycline antibiotic activity in water by stable cubic phase barium stannate-perovskite nanoparticles under energy-efficient blue light LED irradiation
Article Snippet: .. Antibacterial activity of TC (10 mg L −1 ) before and after treatment by BSO/LED system was assessed by the agar well diffusion method (Clinical and Laboratory Standards Institute, CLSI) as per standard protocol. ..

Article Title: Antibacterial activity of green synthesized zinc oxide nanoparticles using Washingtonia robusta H. Wendl fruit extract
Article Snippet: Bacterial infections remain a critical global health concern, often leading to prolonged selfmedication, extended healing times, treatment failures, increased hospitalization, and higher healthcare costs, alongside increased mortality rates.. Recent research efforts have focused on enhancing human health and environmental sustainability through innovative synthesis techniques.. This study presents a straightforward and cost-effective method for the green synthesis of zinc oxide nanoparticles (ZnO NPs) using fruit extract from Washingtonia robusta H. Wendl and investigated their antibacterial activity.

Article Title: Comparison of Antibacterial Efficacy of Triple Antibiotic-Loaded Hydrogel Versus Modified Triple Antibiotic-Loaded Hydrogel as Intracanal Medicament Against Enterococcus faecalis : An In vitro Study
Article Snippet: .. The agar well diffusion method was performed according to the guidelines set forth by the Clinical and Laboratory Standards Institute to evaluate the antibacterial activity against E. faecalis ( ). .. Using a sterile swab, equal amounts of E. faecalis strains were swabbed onto Mueller-Hinton Agar plates (Hi-Media, Mumbai, India.)

Article Title: Antibacterial and cytotoxic activities of a newly green synthesized ZnO/Se nanocomposite combined with Washingtonia robusta H. Wendl fruit extract
Article Snippet: Bacterial infection is one of the most frequent and crucial issues worldwide.. It can prolong the use of self-medication and the healing period or it may be associated with treatment failure that leads to additional hospitalization, increased costs of care and mortality.. Novelties and discoveries are directed toward supporting human health and maintaining the environment through innovative synthesis techniques that are modernized.

Article Title: Exploring antimicrobial properties and oral health benefits of salix subserrata willd: unveiling the potential beyond its use as traditional chewing stick.
Article Snippet: .. Antibacterial agar well diffusion assay The antibacterial activity of the crude extract was primarily evaluated qualitatively using an agar well diffusion method at various test doses (mg/mL) based on available plant mass, The Clinical and Laboratory Standards Institute guideline (CLSI M100) [52] was followed with minor adjustments to fit the current experimental setup. ..

Diffusion-based Assay:

Article Title: Evaluation of Biological Potential of Red Onion Skin Extract for Anticancer and Antimicrobial Activities
Article Snippet: Chemotherapy, while effective in combating cancer, frequently induces severe adverse effects and lacks specificity.. This investigation aims to assess the potential of red onion skin, a byproduct abundant in polyphenols and flavonoids, as a reservoir of natural compounds possessing anticancer and antimicrobial properties.. Two extracts, specifically ethanolic red onion skin (EROS) extract, and chromatographic red onion skin (CROS) extract were subjected to comprehensive biological analysis.

Article Title: Evaluation of Antibacterial Activity of Trichilia dregeana Sond. (Meliaceae) Methanolic Extract Against Gram-Positive and Gram-Negative Bacteria.
Article Snippet: .. The antibacterial activities of the crude extract of Trichilia dregeana Sond. were evaluated using the agar well diffusion method (Clinical and Laboratory Standards Institute 2018). ..

Article Title: Evaluation of Antibacterial Activity of Trichilia dregeana Sond. (Meliaceae) Methanolic Extract Against Gram‐Positive and Gram‐Negative Bacteria
Article Snippet: .. The antibacterial activities of the crude extract of Trichilia dregeana Sond. were evaluated using the agar well diffusion method (Clinical and Laboratory Standards Institute ). ..

Article Title: Removal of tetracycline antibiotic activity in water by stable cubic phase barium stannate-perovskite nanoparticles under energy-efficient blue light LED irradiation
Article Snippet: .. Antibacterial activity of TC (10 mg L −1 ) before and after treatment by BSO/LED system was assessed by the agar well diffusion method (Clinical and Laboratory Standards Institute, CLSI) as per standard protocol. ..

Article Title: Antibacterial activity of green synthesized zinc oxide nanoparticles using Washingtonia robusta H. Wendl fruit extract
Article Snippet: Bacterial infections remain a critical global health concern, often leading to prolonged selfmedication, extended healing times, treatment failures, increased hospitalization, and higher healthcare costs, alongside increased mortality rates.. Recent research efforts have focused on enhancing human health and environmental sustainability through innovative synthesis techniques.. This study presents a straightforward and cost-effective method for the green synthesis of zinc oxide nanoparticles (ZnO NPs) using fruit extract from Washingtonia robusta H. Wendl and investigated their antibacterial activity.

Article Title: Comparison of Antibacterial Efficacy of Triple Antibiotic-Loaded Hydrogel Versus Modified Triple Antibiotic-Loaded Hydrogel as Intracanal Medicament Against Enterococcus faecalis : An In vitro Study
Article Snippet: .. The agar well diffusion method was performed according to the guidelines set forth by the Clinical and Laboratory Standards Institute to evaluate the antibacterial activity against E. faecalis ( ). .. Using a sterile swab, equal amounts of E. faecalis strains were swabbed onto Mueller-Hinton Agar plates (Hi-Media, Mumbai, India.)

Article Title: Antibacterial and cytotoxic activities of a newly green synthesized ZnO/Se nanocomposite combined with Washingtonia robusta H. Wendl fruit extract
Article Snippet: Bacterial infection is one of the most frequent and crucial issues worldwide.. It can prolong the use of self-medication and the healing period or it may be associated with treatment failure that leads to additional hospitalization, increased costs of care and mortality.. Novelties and discoveries are directed toward supporting human health and maintaining the environment through innovative synthesis techniques that are modernized.

Article Title: Exploring antimicrobial properties and oral health benefits of salix subserrata willd: unveiling the potential beyond its use as traditional chewing stick.
Article Snippet: .. Antibacterial agar well diffusion assay The antibacterial activity of the crude extract was primarily evaluated qualitatively using an agar well diffusion method at various test doses (mg/mL) based on available plant mass, The Clinical and Laboratory Standards Institute guideline (CLSI M100) [52] was followed with minor adjustments to fit the current experimental setup. ..



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Image Search Results


Journal: ACS Omega

Article Title: Synthesis and Characterization of Luminescent and Antibacterial Europium-Titanate nanotubes

doi: 10.1021/acsomega.5c08930

Figure Lengend Snippet: Antibacterial Activity of the Nanomaterials against S. aureus ATCC 25923 and E. coli ATCC 25922

Article Snippet: The antibacterial properties of TiNts, TiNts/Eu, tta, phen, [Eu (tta) 3 phen], and TiNts [Eu (tta) 3 phen] was first evaluated by the agar well diffusion method against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.

Techniques: Activity Assay, Concentration Assay, Control

Antibacterial activity of TiNts, [Eu­(tta) 3 phen], TiNtsTi/Eu and TiNts­[Eu­(tta) 3 phen] against S. aureus ATCC 25923 and E. coli ATCC 25922. Note: 1 (3 mg mL –1 ), 2 (6 mg mL –1 ), 3 (12 mg mL –1 ), 4 (25 mg mL –1 ). B, corresponds to the negative control (sterilized deionized water); C, corresponds to the gentamicin (10 μg). All tests were performed in triplicate for each bacterium.

Journal: ACS Omega

Article Title: Synthesis and Characterization of Luminescent and Antibacterial Europium-Titanate nanotubes

doi: 10.1021/acsomega.5c08930

Figure Lengend Snippet: Antibacterial activity of TiNts, [Eu­(tta) 3 phen], TiNtsTi/Eu and TiNts­[Eu­(tta) 3 phen] against S. aureus ATCC 25923 and E. coli ATCC 25922. Note: 1 (3 mg mL –1 ), 2 (6 mg mL –1 ), 3 (12 mg mL –1 ), 4 (25 mg mL –1 ). B, corresponds to the negative control (sterilized deionized water); C, corresponds to the gentamicin (10 μg). All tests were performed in triplicate for each bacterium.

Article Snippet: The antibacterial properties of TiNts, TiNts/Eu, tta, phen, [Eu (tta) 3 phen], and TiNts [Eu (tta) 3 phen] was first evaluated by the agar well diffusion method against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.

Techniques: Activity Assay, Negative Control

Antibacterial activity of TiNts­[Eu­(tta) 3 phen] against S. aureus ATCC 25923 and E. coli ATCC 25922. Note: 1 (6 mg mL –1 ), 2 (12 mg mL –1 ), 3 (25 mg mL –1 ), 4 (50 mg mL –1 ), 5 (100 mg mL –1 ).

Journal: ACS Omega

Article Title: Synthesis and Characterization of Luminescent and Antibacterial Europium-Titanate nanotubes

doi: 10.1021/acsomega.5c08930

Figure Lengend Snippet: Antibacterial activity of TiNts­[Eu­(tta) 3 phen] against S. aureus ATCC 25923 and E. coli ATCC 25922. Note: 1 (6 mg mL –1 ), 2 (12 mg mL –1 ), 3 (25 mg mL –1 ), 4 (50 mg mL –1 ), 5 (100 mg mL –1 ).

Article Snippet: The antibacterial properties of TiNts, TiNts/Eu, tta, phen, [Eu (tta) 3 phen], and TiNts [Eu (tta) 3 phen] was first evaluated by the agar well diffusion method against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923.

Techniques: Activity Assay